Cis-dihydroethylcatechol dehydrogenase

In enzymology, a cis-dihydroethylcatechol dehydrogenase (EC 1.3.1.66) is an enzyme that catalyzes the chemical reaction

cis-1,2-dihydro-3-ethylcatechol + NAD⁺ ${\displaystyle \rightleftharpoons }$ 3-ethylcatechol + NADH + H⁺

Thus, the two substrates of this enzyme are cis-1,2-dihydro-3-ethylcatechol and NAD⁺, whereas its 3 products are 3-ethylcatechol, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is cis-1,2-dihydro-3-ethylcatechol:NAD+ oxidoreductase. This enzyme participates in ethylbenzene degradation.

• Gibson DT, Gschwendt B, Yeh WK, Kobal VM (1973). "Initial reactions in the oxidation of ethylbenzene by Pseudomonas putida". Biochemistry. 12 (8): 1520–8. doi:10.1021/bi00732a008. PMID 4699984.